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磁微粒酶联免疫吸附法测定玉米中的伏马毒素B1

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2017-06-07 来源:食品科学网
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  作者:管 笛,潘灿平,王 文,武会娟

  关键词:magnetic particles; enzyme-linked immunosorbent assay; fumonisins; corn; protein A

  内容:Magnetic particles (MPs) were used as the support to replace enzyme-linked immunosorbent assay (ELISA) plates for
the determination of fumonisin B1 (FB1) in corn. Goat anti-rabbit antibody and protein A-MP conjugates were utilized in a direct
competitive ELISA format. Under optimized conditions, the limits of detection (LOD) for goat anti-rabbit antibody and protein
A-MPs were 0.024 and 0.030 μg/mL, respectively. The goat anti-rabbit antibody MP conjugates exhibited a lower LOD which
was only one third of that of conventional ELISA. The recoveries from spiked corn varied from 76.4% to 110.6%, with relative
standard deviation (RSD) less than 11%. Overall, a sensitive and stable method for FB1 has been developed in this paper.

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