领学术科研之先，创食品科技之新
—— 中国食品杂志社
Lacto-N-neotetraose (LNnT) is a crucial neutral core human milk oligosaccharide (HMO). In this study, we established a LNnT-producing Saccharomyces cerevisiae cell factory through comprehensive metabolic engineering. Specifically, the de novo biosynthetic pathway of LNnT was assembled by heterologously expressing the lactose permease (lac12) from Kluyveromyces lactis and the glycosyltransferase from Neisseria meningitidis in S. cerevisiae. Subsequently, carbon source regulation based on the glucose-sensitive GAL regulatory system was employed to optimize the expression time of heterologous genes, achieving a production of 15.61 mg/L of LNnT in shake-flask fermentation. In addition, the key rate-limiting steps involved in LNnT synthesis pathway were identified and the corresponding genes were overexpressed to enhance LNnT production, resulting in an 8-fold increase in LNnT titer compared to that of parental strain. To our knowledge, this is the first report on LNnT biosynthesis in S. cerevisiae, opening up the possibility of green production of LNnT using food-safe microorganisms.
电话: 010-87293157
地址: 北京市丰台区洋桥70号
版权所有 @ 2023 中国食品杂志社 京公网安备11010602060050号 京ICP备14033398号-2
