Nonalcoholic fatty liver disease (NAFLD) is a liver disorder causes people who drink little or no alcohol to accumulate extra fat in their livers. It’s getting prevalent in those who are obese. This study was aimed to investigate effects of the consumption of bread fortified with lupin seeds (LS) and psyllium husk (PSH) on NAFLD. Forty-two male rats were divided into 6 groups of 7 animals including 2 groups served as negative controls were given a basal diet and another a basal diet supplemented with unfortified bread. While, the rest of rats (n = 28) were administration with 25% fructose dissolved in water for 8 weeks to induce NAFLD. Then reclassify into 4 subgroups, only one fed on basal diet containing unfortified bread served as positive control group and three subgroups fed on bread fortified with 10% of (LS or PSH or their mixture) powder/kg diet respectively, for 28 days. The results observed an improvement in the nutritious qualities of bread with addition of (LS or PSH), especially in terms of proteins, fiber, total phenolic compounds (TPC) and antioxidant (AA). Bread with 10% LS showed highest (TPC and AA), which recorded (2.96 mg GAE/g and 78.15%), while the flavonoids showed highest value in 10% (LS + PHS) bread. Biological results all treated rat groups revealed improve in body weight, lipid profile and glycemic indices. Similarly, bread fortified with LS and PSH reduced liver (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase) and kidney function (uric acid, urea, creatinine). Interestingly, LS and PSH effectively prevented the increase thiobarbituric acid reactive substances value, but increased levels of reduced glutathione, superoxide dismutase and catalase. Also effectively protected liver from histological changes. Conclusion, LS or PSH is excellent resources of natural antioxidants that might be added to functional foods an important agent for the protection from NAFLD.