Background: Vitis vinifera is universally appreciated herb (especially fruit) for their delicacy, nutrition and conventional as functional food. In the present study, we evaluated anti-hypercholesterolemic potential of Vitis vinifera red leaves extract in experimental wistar male rats. Material and methods: Qualitative and quantitative phytochemical screening of vitis vinifera methanolic extract (VVME) and vitis vinifera aqueous extract aqueous (VVAE) extract was carried out to identify the phytoconstituents. The anti-hypercholesterolemic activity was evaluated by inducing hypercholesterolemia with high cholesterol diet for 21 days in experimental animals. During the experimental periods parameters like lipid profile, liver function test, atherogenic index and histopathological studies were analysed. Results: Total tannins, total flavonoids and total phenolic contents were found in major amount in VVME. The lipid levels were significantly attenuated by different doses ofVVMEandVVAE. Moreover,VVMEwas found more effective thanVVAE and histopathological results confirmed the effectiveness of VVME.