领学术科研之先，创食品科技之新
—— 中国食品杂志社
in this work, a magnetic molecularly imprinted polymer with good adsorption capacity and a 2-methylimidazole (Mi)-Cu-guanosine 5’-monophosphoric acid (GMP) nanozyme with high catalysis activity were first synthesized. then, using the imprinted polymer as biomimetic antibody and the Mi-Cu-GMP nanozyme as label, a novel direct competitive biomimetic enzyme-linked immunoassay (bELiSa) method was established for the sensitive detection of acrylamide in foods. Under optimal conditions, the limit of detection (LoD, iC15) and sensitivity (iC50) of the bELiSa method towards acrylamide were 0.02 and 4.52 mg/L, respectively. the bELiSa method was successfully applied for acrylamide detection in spike potato crisp samples, with recoveries ranging from 87.50% to 106.67% being obtained. further, the levels of acrylamide in coffee, fried dough twists and biscuit samples were determined by the bELiSa and high performance liquid chromatography, with no significant difference found between the results of the two methods. this work thus introduces a novel, fast and accurate method for acrylamide detection in foods.
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