The low temperature is an effective method for postharvest fruits preservation. However, the ice crystal nucleation caused by low temperature significantly decreases the quality of fruits. In this paper, the effects of alternating magnetic field (AMF) on the ice nucleation temperature of grape were investigated, and the AMF continued treatment and AMF intermittent treatment (20 Gs, 60 Hz) assisted near-freezing temperature (AMCT/AMIT-NFT) were used to store grapes at (−2.5 ± 0.2) °C. Compared with the untreated group, AMF decreased the ice nucleation temperature of grape 1 °C. Meanwhile, Results demonstrated that AMCT/AMIT-NFT storage improved sensory properties and antioxidant capacity of grapes, with the overall effect of AMIT treatment being the most favorable. After 9 days of storage, the color difference and polyphenol oxidase (PPO) activity of AMIT treatment were reduced by 17% and 12%, respectively, compared with the untreated group, and it effectively slowed down malonaldehyde (MDA) accumulation. These conclusions show that AMCT/AMIT-NFT storage is an effective tool for prolonging the shelf life of grapes.